通过腰椎穿刺对胸部脊髓损伤小鼠进行嗅鞘细
2017-7-18 来源：不详 浏览次数：次德孚医药出版社在神经修复学期刊出版发表了最新文章：通过腰椎穿刺对胸部脊髓损伤小鼠进行嗅鞘细胞的鞘内移植
Objectives:Toinvestigatethedistributionandfunctionofolfactoryensheathingcells(OECs)followinglumbarpuncture(LP)transplantationinmousespinalcordinjury(SCI).
Methods:OECsweretransplantedbyLPatlevelL3–5,1weekaftertransectedSCIatT8vertebra.Micewerekilledat3,21,and56daysafterLPtransplantation,andtherelativedistributionofcellsatT8vertebrawasquantitated.Theinjuredspinewasalsotestedbyimmunohistochemistrytoassessneuronregenerationandscarbridgingat8weeksposttransplantation.MotorfunctionsofmicewereevaluatedduringtheobservationperiodusingtheBassoMouseScale.
Results:OECswereexaminedandconfirmedbystudyingcellmorphologyunderphasecontrastandimmunostainingofNGFRp75.LP-transplantedOECscouldbedetectedjust3daysaftertransplantation(p75+area:0.16mm2)andaccumulatedto0.31and0.30mm2at21and56dayspostengraftment,respectively.Thenumberofendogenousneurons,?to+μm,farfromtheepicenter,inOEC-transplantedmicewasmorethanthatinSCImicewithoutengraftment.SCIlesionofmiceinthecontrolgroup(.3±3.97μm)wasmuchlongerthanthatinOEC-graftedgroup(.7±3.60μm).GraftsofOECsinducedsignificantfunctionalimprovementinmicethatunderwentT8vertebraltransection,justfrom3daysaftercellinjection.
Conclusion:LPisaminimallyinvasivemethodforOECtransplantationtotreatSCI.ThisisthefirststudytovisualizethedistributionandfunctionsofLP-transplantedOECsintheintactandinjuredspinalcord.
Keywords:olfactoryensheathingcells,spinalcordinjury,intrathecaltransplantation,lumbarpuncture,neuronregeneration,scarbridging
